Losberger C, Ernst J F
Division of Molecular Biology, Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Curr Genet. 1989 Sep;16(3):153-8. doi: 10.1007/BF00391471.
The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to orotidine-5'-phosphate decarboxylases (ODCases) of other fungal species. The C. albicans ODCase is most closely related to the ODCases of the budding yeasts Kluyveromyces lactis and S. cerevisiae (74% and 71% homology, respectively). Most 5' ends of URA3 transcripts in the authentic host and in the heterologous host S. cerevisiae were found to be identical. These results demonstrate a close taxonomic relationship between non-pathogenic budding yeasts and C. albicans.
人类病原体白色念珠菌以酵母形式或丝状形式生长。我们已经确定了一个1.365 kb的白色念珠菌基因组片段的序列，该片段可互补酿酒酵母ura3和大肠杆菌pyrF突变。该片段内的一个开放阅读框对应于一个270个氨基酸的蛋白质，该蛋白质与其他真菌物种的乳清苷-5'-磷酸脱羧酶（ODCases）具有同源性。白色念珠菌ODCase与出芽酵母乳酸克鲁维酵母和酿酒酵母的ODCases关系最为密切（分别具有74%和71%的同源性）。发现在天然宿主和异源宿主酿酒酵母中URA3转录本的大多数5'末端是相同的。这些结果表明非致病性出芽酵母和白色念珠菌之间存在密切的分类学关系。
