Osterlund A
Department of Clinical Microbiology, University Hospital, Uppsala, Sweden.
Scand J Infect Dis. 1995;27(2):131-4. doi: 10.3109/00365549509018992.
Arcanobacterium haemolyticum is an infrequent agent of pharyngotonsillitis in children and young adults. Despite the fact that A. haemolyticum is fully sensitive to penicillin in vitro, penicillin treatment failures are frequent. The ability of A. haemolyticum to invade HEp-2 cells and survive intracellulary was investigated. All 12 strains tested, of which 10 were isolated from patients with pharyngotonsillitis, and 2 were reference strains, were internalized by the HEp-2 cells. Four strains tested further, one of the reference strains and 3 of the clinical isolates, proved able to survive intracellularly for 4 days, thus creating intracellular reservoirs of bacteria. It was also shown that erythromycin, an antibiotic known to penetrate well intracellularly, efficiently killed these bacteria.
溶血隐秘杆菌是儿童和青年咽扁桃体炎的一种少见病原体。尽管溶血隐秘杆菌在体外对青霉素完全敏感，但青霉素治疗失败却很常见。对溶血隐秘杆菌侵袭人喉表皮癌细胞（HEp-2细胞）并在细胞内存活的能力进行了研究。所检测的12株菌株，其中10株从咽扁桃体炎患者中分离得到，2株为参考菌株，均被HEp-2细胞内化。进一步检测的4株菌株，其中1株参考菌株和3株临床分离株，被证明能够在细胞内存活4天，从而形成细菌的细胞内储存库。还表明，红霉素这种已知能有效穿透细胞的抗生素，能有效杀灭这些细菌。
