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利用巨片形吸虫成虫的一种28 kDa半胱氨酸蛋白酶对反刍动物肝片吸虫病进行免疫诊断。

Immunodiagnosis of fasciolosis in ruminants using a 28-kDa cysteine protease of Fasciola gigantica adult worms.

作者信息

Fagbemi B O, Guobadia E E

机构信息

Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.

出版信息

Vet Parasitol. 1995 Apr;57(4):309-18. doi: 10.1016/0304-4017(94)00684-5.

DOI:10.1016/0304-4017(94)00684-5
PMID:7660568
Abstract

A homogeneous extract of a 28-kDa cysteine protease of Fasciola gigantica adult worms was used as the antigen for immunodiagnosis of fasciolosis in cattle, sheep and goats using the Falcon assay screening test enzyme-linked immunosorbent assay technique. This antiprotease assay technique was found to be very rapid and sensitive and may be useful as a supplementary method for the diagnosis of fasciolosis in ruminants.

摘要

用巨片形吸虫成虫的一种28 kDa半胱氨酸蛋白酶的匀浆提取物作为抗原,采用FAS Falcon检测筛选试验酶联免疫吸附测定技术对牛、绵羊和山羊的片形吸虫病进行免疫诊断。发现这种抗蛋白酶检测技术非常快速且灵敏,可作为反刍动物片形吸虫病诊断的一种补充方法。

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