Frøen J F, Larsen T H
Department of Anatomy and Cell Biology, University of Bergen, Norway.
Acta Anat (Basel). 1995;152(2):119-26. doi: 10.1159/000147690.
Using confocal microscopy and immunocytochemistry we have studied early changes in distribution of fibronectin (FN) in myocardial cells of rats subjected to experimental acute myocardial ischemia (AMI) by coronary ligation for several periods of 0.5 h to 6 days. In sham-operated and nonoperated rats, FN was present in the interstitium around the myocytes, and in their transverse tubules (TT). Already after 0.5 h of ischemia there was a well-defined increase of immunoreactive FN in focal areas of the interstitium of the hypoperfused portion, and distinct penetration into adjacent myocytes. The early penetration of FN into myocytes appears to follow a path through the TT, with a codistribution with actin in the I bands. This process precedes a total and diffuse infiltration of FN into the cytoplasm of disintegrating myocytes at later stages of coronary occlusion.
我们运用共聚焦显微镜和免疫细胞化学技术，研究了通过冠状动脉结扎造成实验性急性心肌缺血（AMI）的大鼠心肌细胞中纤连蛋白（FN）分布的早期变化，结扎时长从0.5小时至6天不等。在假手术和未手术的大鼠中，FN存在于心肌细胞周围的间质以及横管（TT）中。缺血0.5小时后，灌注不足部分的间质局部区域免疫反应性FN就明显增加，并明显渗透到相邻的心肌细胞中。FN早期渗透到心肌细胞似乎是通过TT途径，在I带与肌动蛋白共分布。这个过程先于冠状动脉闭塞后期FN全面、弥漫性地浸润到解体的心肌细胞胞质中。
