甘薯羽状斑驳病毒强毒株（SPFMV-S）基因组RNA的两个核内含体和外壳蛋白基因的核苷酸序列分析

Nucleotide sequence analysis of two nuclear inclusion body and coat protein genes of a sweet potato feathery mottle virus severe strain (SPFMV-S) genomic RNA.

作者信息

Mori M, Sakai J, Kimura T, Usugi T, Hayashi T, Hanada K, Nishiguchi M

机构信息

Kyushu National Agricultural Experiment Station, Kumamoto, Japan.

出版信息

Arch Virol. 1995;140(8):1473-82. doi: 10.1007/BF01322674.

DOI:10.1007/BF01322674

PMID:7661699

Abstract

Recombinant DNA molecules containing cDNA of a sweet potato feathery mottle virus severe strain (SPFMV-S) RNA genome were constructed and the partial nucleotide sequences were determined for three DNA inserts, which cover 4-2 kb from the 3'-terminus excluding the poly (A) tail. This region of the genome consists of an open reading frame of 1340 amino acids (a.a.) and a 3'-non-translated region of 224 nucleotides. The protein products expected were 6K2 (53 a.a.), NIa (435 a.a.), NIb (521 a.a.) and CP (315 a.a.). Among NIa, NIb and coat proteins, the NIb protein was found to be the most conserved (59-68%) when compared to the corresponding proteins of other distinct potyviruses.

摘要

构建了含有甘薯羽状斑驳病毒强毒株（SPFMV-S）RNA基因组cDNA的重组DNA分子，并测定了三个DNA插入片段的部分核苷酸序列，这些片段从3'-末端起覆盖4 - 2 kb（不包括聚腺苷酸尾）。基因组的该区域由一个1340个氨基酸（a.a.）的开放阅读框和一个224个核苷酸的3'-非翻译区组成。预期的蛋白质产物为6K2（53个a.a.）、NIa（435个a.a.）、NIb（521个a.a.）和CP（315个a.a.）。在NIa、NIb和外壳蛋白中，与其他不同马铃薯Y病毒属病毒的相应蛋白相比，NIb蛋白被发现是最保守的（59 - 68%）。