Tanaka H, Watanabe T
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Japan.
J Ind Microbiol. 1995 Jun;14(6):478-83. doi: 10.1007/BF01573962.
Lysis of cell walls of various yeast species by beta-1.3- and beta-1,6-glucanases of Bacillus circulans WL-12 was investigated. Selective enzymolysis of cell walls of Pyricularia oryzae by single and combined actions of beta-1,3-,beta-1,6-glucanases and chitinase was followed. Chemical structure of the cell wall glucan of P. oryzae was determined by chemical and enzymatic methods. Multiple component nature of glucanases of B. circulans WL-12, their induction and lytic actions on cell walls of various yeasts were studied. Genes specifying glucanases and chitinases of B. circulans WL-12 were cloned in E. coli, and their nucleotide sequences were determined. Fibronectin type III modules were found in the chitinases. Functions of the domains of the deduced structures of the glucanases and the chitinases were studied by various methods including molecular genetic techniques.
研究了环状芽孢杆菌WL - 12的β - 1,3 - 葡聚糖酶和β - 1,6 - 葡聚糖酶对各种酵母细胞壁的裂解作用。跟踪了β - 1,3 - 葡聚糖酶、β - 1,6 - 葡聚糖酶和几丁质酶单独及联合作用对稻瘟病菌细胞壁的选择性酶解。通过化学和酶学方法确定了稻瘟病菌细胞壁葡聚糖的化学结构。研究了环状芽孢杆菌WL - 12葡聚糖酶的多组分性质、它们的诱导作用以及对各种酵母细胞壁的裂解作用。将环状芽孢杆菌WL - 12的葡聚糖酶和几丁质酶基因克隆到大肠杆菌中，并测定了它们的核苷酸序列。在几丁质酶中发现了纤连蛋白III型结构域。通过包括分子遗传学技术在内的各种方法研究了葡聚糖酶和几丁质酶推导结构域的功能。
