枯草芽孢杆菌分泌型β-葡聚糖酶的基因及遗传控制位点的DNA序列。
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
作者信息
Murphy N, McConnell D J, Cantwell B A
出版信息
Nucleic Acids Res. 1984 Jul 11;12(13):5355-67. doi: 10.1093/nar/12.13.5355.
Abstract
The sequence of a 1409 base pair restriction fragment containing the B. subtilis gene for (1-3), (1-4)-beta-D-glucan endoglucanase is reported. The gene is encoded in a 726 base pair segment. The deduced amino acid sequence of the protein has a hydrophobic signal peptide at the NH2-terminus similar to those found in five other secreted proteins from Bacillus. The gene is preceded by a sequence resembling promoters for the vegetative B. subtilis RNA polymerase. This is followed by a sequence resembling a B. subtilis ribosome binding site nine nucleotides before the first codon of the gene. Two sequences, one before and one after the gene, can be arranged in secondary structures similar to transcriptional terminators. There is also a short open reading frame coding for a hydrophobic protein on the minus strand just upstream from the beta-glucanase gene. A possible role for this gene in the control of expression of beta-glucanase is suggested.
摘要
报道了包含枯草芽孢杆菌(1-3),(1-4)-β-D-葡聚糖内切葡聚糖酶基因的一个1409个碱基对的限制性片段的序列。该基因编码于一个726个碱基对的区段。推导的该蛋白质氨基酸序列在NH2末端有一个疏水信号肽，类似于在枯草芽孢杆菌的其他五种分泌蛋白中发现的信号肽。该基因之前有一段类似于枯草芽孢杆菌营养型RNA聚合酶启动子的序列。在该基因的第一个密码子之前九个核苷酸处，接着是一段类似于枯草芽孢杆菌核糖体结合位点的序列。该基因前后各有一段序列，可形成类似于转录终止子的二级结构。在β-葡聚糖酶基因上游的负链上，还有一个编码疏水蛋白的短开放阅读框。提出了该基因在β-葡聚糖酶表达调控中的可能作用。
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