Hodrová B, Kopecný J, Petr O
Institute of Animal Physiology and Genetics, Academy of Sciences, Prague, Czech Republic.
Lett Appl Microbiol. 1995 Jul;21(1):34-7. doi: 10.1111/j.1472-765x.1995.tb01001.x.
The degradation and fermentation of microcrystalline cellulose were studied in monoculture of the polycentric anaerobic fungus Orpinomyces joyonii and in co-cultures with the rumen bacteria Megasphaera elsdenii and Eubacterium limosum. More than 25% of cellulose hydrolysis products (glucose and cellodextrins) were released by the fungus into the medium after 8 d of cultivation. These products were metabolized by bacteria in mixed cultures. In co-culture with the fungus M. elsdenii and E. limosum increased the extent of microcrystalline cellulose degradation by 10.12% and 7.96%, respectively. Biomass yield in co-cultures was increased by 89.9% and 59.4% for M. elsdenii and E. limosum. Y cellulose for fungus alone was 52.29 g dry matter mol-1 glucose. These values were 64.93 and 55.92 g mol-1 glucose unit in co-culture with M. elsdenii and E. limosum, respectively.
在多中心厌氧真菌约氏奥皮那菌的纯培养以及与瘤胃细菌埃氏巨球形菌和黏液真杆菌的共培养中，研究了微晶纤维素的降解和发酵。培养8天后，真菌将超过25%的纤维素水解产物（葡萄糖和纤维糊精）释放到培养基中。这些产物在混合培养物中被细菌代谢。与真菌共培养时，埃氏巨球形菌和黏液真杆菌分别使微晶纤维素的降解程度提高了10.12%和7.96%。埃氏巨球形菌和黏液真杆菌共培养时的生物量产量分别提高了89.9%和59.4%。单独培养真菌时，每摩尔葡萄糖的纤维素产率为52.29克干物质。与埃氏巨球形菌和黏液真杆菌共培养时，这些值分别为每摩尔葡萄糖单位64.93克和55.92克。
