Wigley D B
Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
Annu Rev Biophys Biomol Struct. 1995;24:185-208. doi: 10.1146/annurev.bb.24.060195.001153.
DNA topoisomerases are ubiquitous enzymes that control the level of supercoiling of DNA in cells. There are several classes, each with distinct properties, which are briefly discussed in this review. High-resolution X-ray crystallographic structures have been obtained for fragments of two classes of these enzymes, which when combined with biochemical data, reveal a great deal about the gymnastics that the enzymes undergo during catalysis and provide fascinating snapshots of their mechanisms. These mechanisms are discussed in detail. Finally, the first structure of a topoisomerase in a complex with an antibiotic was recently solved. This structure is briefly discussed with regard to the biochemical activity of the compound.
DNA拓扑异构酶是细胞中普遍存在的一类酶，可控制DNA的超螺旋水平。拓扑异构酶分为几类，每一类都有不同的特性，本文将对其进行简要讨论。现已获得两类此类酶片段的高分辨率X射线晶体结构，这些结构与生化数据相结合，揭示了很多关于酶在催化过程中所经历的复杂变化，并提供了其作用机制的精彩瞬间。本文将详细讨论这些机制。最后，最近解析了拓扑异构酶与一种抗生素复合物的首个结构。本文将针对该化合物的生化活性对这一结构进行简要讨论。
