Diesfeld H J, Kirsten C
Tropenmed Parasitol. 1975 Dec;26(4):499-502.
The localization of the antigen-antibody reactions on methacrylate embedded sections of an adult male and female of the nematode Dipetalonema vitae by the indirect immunofluorescence test was found to be as follows: female D. vitae: in the egg-shell and circumference of sections of the microfilariae as well as amorphous structures in the vesica seminalis and vas deferens. In both sexes: partially in the somatic muscle cells according to their glycogen content, the interstitial spaces between the muscle cells and the connecting pseudocoelomic cavity. Reference to the possible application of this technique in the immunofluorescence diagnosis of filariasis is made.
通过间接免疫荧光试验，发现在甲基丙烯酸酯包埋的成年雄性和雌性丝状线虫双瓣尾丝虫切片上，抗原 - 抗体反应的定位如下：雌性双瓣尾丝虫：在微丝蚴切片的卵壳和周围，以及精囊和输精管中的无定形结构。在两性中：部分根据糖原含量存在于体肌细胞、肌细胞之间的间隙和连接的假体腔中。文中还提及了该技术在丝虫病免疫荧光诊断中的可能应用。
