高效液相色谱法同时测定人血浆中的羟钴胺及其氰化物络合物氰钴胺。大剂量羟钴胺作为严重氰化物中毒解毒剂后的药代动力学研究应用。

Simultaneous determination of hydroxocobalamin and its cyanide complex cyanocobalamin in human plasma by high-performance liquid chromatography. Application to pharmacokinetic studies after high-dose hydroxocobalamin as an antidote for severe cyanide poisoning.

作者信息

Astier A, Baud F J

机构信息

Laboratoire de Toxicologie, CHU Henri Mondor, Créteil, France.

出版信息

J Chromatogr B Biomed Appl. 1995 May 5;667(1):129-35. doi: 10.1016/0378-4347(95)00012-8.

DOI:10.1016/0378-4347(95)00012-8

PMID:7663675

Abstract

Hydroxocobalamin (OHCbl) is a powerful antidote for cyanide poisoning, via the formation of non-toxic cyanocobalamin (CNCbl). Plasmatic cobalamins were measured at 361 nm, after enrichment and purification on a short C18 precolumn (1% acetic acid; 1 ml min-1; 2 min), by back-flush elution on a C18 ODS-2 column [0.1 M sodium dihydrogenphosphate-methanol (63:27, v/v) (pH 4.0); 0.80 ml min-1]. The precision was 3.21 and 3.54% for 10 microM OHCbl and CNCbl, respectively. The method was used to study the pharmacokinetics of OHCbl and the formed CNCbl in severely poisoned patients.

摘要

羟钴胺素（OHCbl）是氰化物中毒的一种强效解毒剂，它通过形成无毒的氰钴胺素（CNCbl）发挥作用。在短C18预柱（1%乙酸；1 ml/min；2 min）上进行富集和纯化后，于361 nm处测定血浆钴胺素，然后通过在C18 ODS-2柱上反冲洗脱[0.1 M磷酸二氢钠-甲醇（63:27，v/v）（pH 4.0）；0.80 ml/min]。对于10 microM的OHCbl和CNCbl，精密度分别为3.21%和3.54%。该方法用于研究OHCbl及其形成的CNCbl在重度中毒患者体内的药代动力学。