Kato H, Terauchi R, Utech F H, Kawano S
Department of Botany, Faculty of Science, Kyoto University, Japan.
Mol Phylogenet Evol. 1995 Jun;4(2):184-93. doi: 10.1006/mpev.1995.1018.
In this paper, we examine the phylogenetic affinities of Trilliaceae sensu lato. DNA sequences of the rbcL gene were analyzed for 8 species from Trilliaceae sensu lato (Trillium kamtschaticum, T. sessile, Daiswa polyphylla, Kinugasa japonica, Paris tetraphylla, Scoliopus bigelovii, S. hallii, and Medeola virginiana) and 10 other species from Liliaceae sensu lato. The analysis indicates that Trilliaceae sensu stricto (Trillium, Daiswa, Kinugasa, and Paris) is monophyletic and that Trilliaceae sensu lato is not monophyletic. Scoliopus and Medeola do not form a clade with Trilliaceae sensu stricto. Instead, they form a clade with Uvulariaceae and Liliaceae sensu stricto, respectively. Relative to the rbcL genes of Trilliaceae sensu stricto, Scoliopus and Medeola displayed approximately 14-24 synonymous substitutions per 100 sites. In similar comparisons with Streptopus and Clintonia (Uvulariaceae), these displayed approximately 1-4 synonymous substitutions per 100 sites. The rbcL gene analysis supports the previous suggestion that Scoliopus and Medeola should be moved from Trilliaceae to Liliaceae sensu stricto. The analysis also indicated that Veratrum (Melanthiaceae) may be related to Trilliaceae sensu stricto. For reconstructing the phylogeny of lower taxonomic level as such, it was found that data on nonsynonymous base substitution as well as weighting in the first and second positions of codons are not useful.
在本文中，我们研究了广义延龄草科的系统发育亲缘关系。分析了广义延龄草科8个物种（东北延龄草、 sessile延龄草、多叶重楼、日本重楼、四叶重楼、大花油点草、哈氏油点草和弗吉尼亚延龄草）以及广义百合科其他10个物种的rbcL基因序列。分析表明，狭义延龄草科（延龄草属、重楼属、日本重楼属和重楼属）是单系的，而广义延龄草科不是单系的。油点草属和延龄草属不与狭义延龄草科形成一个分支。相反，它们分别与狭义的钟花科和百合科形成一个分支。相对于狭义延龄草科的rbcL基因，油点草属和延龄草属每100个位点显示出约14 - 24个同义替换。与扭柄花属和克林顿属（钟花科）的类似比较中，这些每100个位点显示出约1 - 4个同义替换。rbcL基因分析支持了之前的建议，即油点草属和延龄草属应从延龄草科移至狭义百合科。分析还表明藜芦属（藜芦科）可能与狭义延龄草科有关。对于构建这样的低分类水平的系统发育，发现非同义碱基替换的数据以及密码子第一和第二位的加权并不有用。
