结节病中肺泡巨噬细胞肿瘤坏死因子-α释放及支气管肺泡灌洗细胞表型

Alveolar macrophage TNF-alpha release and BAL cell phenotypes in sarcoidosis.

作者信息

Zheng L, Teschler H, Guzman J, Hübner K, Striz I, Costabel U

机构信息

Department of Pneumology and Allergy, Ruhrlandklinik, Medical Faculty, University of Essen, Germany.

出版信息

Am J Respir Crit Care Med. 1995 Sep;152(3):1061-6. doi: 10.1164/ajrccm.152.3.7663784.

DOI:10.1164/ajrccm.152.3.7663784

PMID:7663784

Abstract

The aim of this study was to investigate the relationship between release of tumor necrosis factor-alpha (TNF-alpha) by alveolar macrophages (AM) and the phenotypic characteristics of bronchoalveolar lavage (BAL) cells in sarcoidosis. We studied the spontaneous release of TNF-alpha by AM in vitro and the phenotypic characteristics of freshly recovered BAL T-cells and AM in 31 individuals (13 with active sarcoidosis, nine with inactive sarcoidosis, and nine normal controls). TNF-alpha was measured by enzyme-linked immunosorbent assay (ELISA) in supernatants from unstimulated AM after 24 h culture. Phenotypic markers of BAL cells were determined by an immunocytochemical assay. AM of patients with active sarcoidosis released more TNF-alpha (1,355 +/- 133 pg/ml/ 10(6) AM/24 h) than those of the inactive group (651 +/- 142 pg/ml/10(6) AM/24 h) or the normal controls (425 +/- 121 pg/ml/10(6) AM/24 h), with p < 0.001 for both comparisons. The amount of TNF-alpha released correlated positively with the percentage expression of CD4 (r = 0.72) and CD25 (r = 0.70) by lymphocytes, and of CD14 (r = 0.63), VLA-4 (r = 0.59), FRD1 (r = 0.67) and 27E10 (r = 0.67) by AM, with p < 0.001 for all correlations. In conclusion, this relationship suggests that these antigens may be considered as cellular activation markers, and that some of these AM antigens may indirectly characterize the AM phenotype that is capable of producing TNF-alpha.

摘要

本研究的目的是调查结节病中肺泡巨噬细胞（AM）释放肿瘤坏死因子-α（TNF-α）与支气管肺泡灌洗（BAL）细胞表型特征之间的关系。我们研究了31名个体（13名活动性结节病患者、9名非活动性结节病患者和9名正常对照）的AM体外自发释放TNF-α情况以及新鲜回收的BAL T细胞和AM的表型特征。通过酶联免疫吸附测定（ELISA）检测培养24小时后未刺激的AM上清液中的TNF-α。通过免疫细胞化学测定确定BAL细胞的表型标志物。活动性结节病患者的AM释放的TNF-α（1,355±133 pg/ml/10⁶ AM/24小时）比非活动性组（651±142 pg/ml/10⁶ AM/24小时）或正常对照组（425±121 pg/ml/10⁶ AM/24小时）更多，两组比较p均<0.001。释放的TNF-α量与淋巴细胞CD4（r = 0.72）和CD25（r = 0.70）的表达百分比以及AM的CD14（r = 0.63）、VLA-4（r = 0.59）、FRD1（r = 0.67）和27E10（r = 0.67）的表达百分比呈正相关，所有相关性p均<0.001。总之，这种关系表明这些抗原可被视为细胞活化标志物，并且这些AM抗原中的一些可能间接表征能够产生TNF-α的AM表型。