Soucy D M, Sindlinger J F, Greene S P, Barber A, Illner H, Shires G T
Department of Surgery, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
Crit Care Med. 1995 Sep;23(9):1528-32. doi: 10.1097/00003246-199509000-00013.
Excessive blood loss in a rat model of uncontrolled hemorrhage has been attributed to the vasodilatory effects of a droperidol-ketamine mixture used for anesthesia. The present study compared responses to droperidol-ketamine and pentobarbital with those responses of a control group without anesthesia during uncontrolled hemorrhage.
Prospective, randomized, controlled trial.
University surgical research laboratory.
Forty-five female Sprague-Dawley rats (210 to 275 g).
Rats were randomly divided into three groups of 15 each according to the type of anesthesia: unanesthetized; pentobarbital; and droperidol-ketamine. A 75% tail resection was used to initiate hemorrhage. Mortality, survival time, and blood loss were monitored, and the differences between all three groups were tested using chi 2 test (mortality) and one-way analysis of variance (ANOVA) (survival and blood loss) for statistical significance.
Mean blood loss amounts at 15 mins were 8.9, 13.6, and 22.4 mL/kg for the unanesthetized, pentobarbital, and droperidol-ketamine groups, respectively. Mortality rates for the three groups were 0%, 0%, and 53% at 30 mins and 60%, 33%, and 93% at 90 mins, respectively. Mean survival times for these groups were 94, 135, and 39 mins, respectively.
An excessive rate of blood loss due to the use of droperidol-ketamine anesthesia renders this model inappropriate for investigation of uncontrolled hemorrhage. The response of rats under pentobarbital anesthesia more closely approximates that of unanesthetized rats. However, the higher mortality rate despite the lesser hemorrhage observed in the latter group seems to indicate the existence of other factors (in addition to blood loss) that may contribute to the early death of these animals.
在大鼠失血性休克模型中，麻醉用氟哌利多-氯胺酮混合物的血管舒张作用被认为是导致失血过多的原因。本研究比较了在失血性休克期间，氟哌利多-氯胺酮和戊巴比妥组与未麻醉对照组的反应。
前瞻性、随机、对照试验。
大学外科研究实验室。
45只雌性斯普拉格-道利大鼠（210至275克）。
根据麻醉类型，将大鼠随机分为三组，每组15只：未麻醉组；戊巴比妥组；氟哌利多-氯胺酮组。通过切除75%的尾巴引发出血。监测死亡率、存活时间和失血量，并使用卡方检验（死亡率）和单因素方差分析（存活时间和失血量）检验三组之间的差异，以确定统计学意义。
未麻醉组、戊巴比妥组和氟哌利多-氯胺酮组在15分钟时的平均失血量分别为8.9、13.6和22.4毫升/千克。三组在30分钟时的死亡率分别为0%、0%和53%，在90分钟时分别为60%、33%和93%。这些组的平均存活时间分别为94、135和39分钟。
使用氟哌利多-氯胺酮麻醉导致的失血过快使得该模型不适用于失血性休克的研究。戊巴比妥麻醉下大鼠的反应更接近未麻醉大鼠。然而，尽管后一组观察到的失血量较少，但死亡率较高，这似乎表明存在其他因素（除失血外）可能导致这些动物过早死亡。
