Heinrichs V, Baker B S
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
EMBO J. 1995 Aug 15;14(16):3987-4000. doi: 10.1002/j.1460-2075.1995.tb00070.x.
The SR proteins represent a family of splicing factors several of which have been implicated in the regulation of sex-specific alternative splicing of doublesex (dsx) pre-mRNA in Drosophila. The dsx gene is involved in Drosophila sex determination. We have identified two RNA target sequence motifs recognized by the SR protein RBP1 from Drosophila using an in vitro selection approach. Several copies of these RBP1 target sequences were found within two regions of the dsx pre-mRNA which are important for the regulation of dsx alternative splicing, the repeat region and the purine-rich polypyrimidine tract of the regulated female-specific 3' splice site. We show that RBP1 target sequences within the dsx repeat region are required for the efficient splicing of dsx pre-mRNA. Moreover, our studies reveal that RBP1 contributes to the activation of female-specific dsx splicing in vivo by recognizing the RBP1 target sequences within the purine-rich polypyrimidine tract of the female-specific 3' splice site.
SR蛋白代表了一类剪接因子，其中几种与果蝇中双性（dsx）前体mRNA的性别特异性可变剪接调控有关。dsx基因参与果蝇的性别决定。我们使用体外筛选方法鉴定了果蝇SR蛋白RBP1识别的两个RNA靶序列基序。在dsx前体mRNA的两个区域中发现了这些RBP1靶序列的多个拷贝，这两个区域对dsx可变剪接的调控很重要，即重复区域和受调控的雌性特异性3'剪接位点的富含嘌呤的多嘧啶序列。我们表明，dsx重复区域内的RBP1靶序列是dsx前体mRNA有效剪接所必需的。此外，我们的研究表明，RBP1通过识别雌性特异性3'剪接位点富含嘌呤的多嘧啶序列内的RBP1靶序列，在体内促进雌性特异性dsx剪接的激活。
