Jeannin P, Delneste Y, Life P, Gauchat J F, Kaiserlian D, Bonnefoy J Y
Glaxo Institute for Molecular Biology, Plan les Ouates/Geneva, Switzerland.
Eur J Immunol. 1995 Aug;25(8):2247-52. doi: 10.1002/eji.1830250820.
Interleukin (IL)-12 is a potent inducer of cell-mediated immunity: it favors the generation of interferon (IFN)-gamma-producing T cells, increases IFN-gamma production by T cells and natural killer cells and prevents the generation of a Th2 response in murine in vivo models. Nevertheless, the effects of IL-12 on an established Th2 response remain poorly documented. In the present paper, we analyzed the effect of IL-12 on the profile of lymphokines produced by established IL-4-producing Th0 and Th2-like human T cell clones (TCC) and by polyclonal T cells. We found that IL-12 (i) enhances, as previously reported, IFN-gamma production by Th0 TCC and, to a smaller extent, by Th2-like TCC, (ii) increases the proliferation of Th0 and Th2-like TCC and (iii) unexpectedly, synergizes with T cell receptor-associated or nonspecific stimuli in increasing IL-4 production by these TCC. Thus, IL-12 potentiates the production of IFN-gamma and also of IL-4 by established IL-4 producing TCC. Although IL-12 has been widely reported to induce a Th1 response and to prevent the development of a Th2 response in vivo, IL-12 may on the contrary potentiate an established Th2 response in humans.
白细胞介素（IL）-12是细胞介导免疫的强效诱导剂：它有利于产生干扰素（IFN）-γ的T细胞的生成，增加T细胞和自然杀伤细胞产生的IFN-γ，并在小鼠体内模型中阻止Th2反应的产生。然而，IL-12对已建立的Th2反应的影响仍鲜有文献记载。在本文中，我们分析了IL-12对已建立的产生IL-4的Th0和Th2样人T细胞克隆（TCC）以及多克隆T细胞产生的淋巴因子谱的影响。我们发现，IL-12（i）如先前报道的那样增强了Th0 TCC产生的IFN-γ，在较小程度上也增强了Th2样TCC产生的IFN-γ，（ii）增加了Th0和Th2样TCC的增殖，（iii）出乎意料的是，在增加这些TCC产生IL-4方面，它与T细胞受体相关或非特异性刺激协同作用。因此，IL-12增强了已建立的产生IL-4的TCC产生IFN-γ以及IL-4的能力。尽管广泛报道IL-12在体内诱导Th1反应并阻止Th2反应发展，但相反，IL-12可能会增强人体中已建立的Th2反应。
