Domanski P, Witte M, Kellum M, Rubinstein M, Hackett R, Pitha P, Colamonici O R
Department of Pathology, University of Tennessee, Memphis 38163, USA.
J Biol Chem. 1995 Sep 15;270(37):21606-11. doi: 10.1074/jbc.270.37.21606.
The interferon alpha beta receptor (IFN alpha R) or type I IFN-R is formed by a 110-kDa alpha subunit or IFNAR and by a beta subunit, which has short and long forms (molecular masses of 55 and 95-100 kDa, respectively). In this report, we demonstrate that the IFN alpha/beta R cDNA recently cloned corresponds to the 55-kDa or short form of the beta subunit, while the 95-100-kDa species reported here corresponds to a longer form of the IFN alpha/beta R cDNA that is probably produced by alternative splicing of the same gene. Stable transfection of the alpha subunit with either form of the beta subunit results in the expression of low and high affinity receptors, while expression of either form of the beta subunit alone only produces low affinity receptors. More important, only expression of the alpha and long form of the human beta subunits in mouse L-929 cells reconstitutes the activation of the Jak kinases and the Stat factors, as well as the antiviral response to human type I IFNs.
干扰素αβ受体（IFNαR）或I型干扰素受体由一个110 kDa的α亚基或IFNAR以及一个β亚基组成，该β亚基有短和长两种形式（分子量分别为55 kDa和95 - 100 kDa）。在本报告中，我们证明最近克隆的IFNα/βR cDNA对应于55 kDa的β亚基短形式，而此处报道的95 - 100 kDa的蛋白对应于IFNα/βR cDNA的更长形式，它可能是由同一基因的可变剪接产生的。α亚基与任何一种形式的β亚基稳定转染都会导致低亲和力和高亲和力受体的表达，而单独表达任何一种形式的β亚基只会产生低亲和力受体。更重要的是，只有在小鼠L - 929细胞中表达人α亚基和β亚基的长形式才能重建Jak激酶和Stat因子的激活以及对人I型干扰素的抗病毒反应。
