Maass M, Dalhoff K
Institute of Medical Microbiology, Medical University of Lübeck, Germany.
J Clin Microbiol. 1995 Jul;33(7):1793-6. doi: 10.1128/jcm.33.7.1793-1796.1995.
Chlamydia pneumoniae is characterized by rapidly decreasing viability outside the host cell, and efficient preservation of its infectivity is a prerequisite for subsequent cell culture recovery. Extracellular survival of three C. pneumoniae stock strains and three wild-type strains subjected to simulated conditions of transport was therefore examined in order to establish recommendations for transport and storage of clinical specimens. The presence of fetal calf serum in transport media as well as refrigeration distinctly improved chlamydial retrieval during prolonged transport. Loss of infectivity was kept to a minimum in Eagle's minimal essential medium or sucrose-phosphate-glutamine medium. Storage at 22 degrees C permitted a stock strain recovery of 81% after 12 h. When refrigeration to 4 degrees C was provided, recovery rates of 74% could be achieved after 48 h. Though the strains were from different geographic regions, requirements for good survival were comparable and should therefore apply worldwide. The results indicate that the laboratory strains are not extremely labile. However, comparative examination of the wild-type strains showed less stability: primary isolates were not satisfactorily retrievable beyond 4 h at 22 degrees C or beyond 24 h at 4 degrees C. Further extension of storage times resulted in rapidly decreasing recovery, indicating a requirement to freeze samples at -75 degrees C to preserve viability. Adherence to the shorter storage periods suggested by the data obtained with primary isolates is recommended to ensure successful transport until more extensive testing with clinical materials is available.
肺炎衣原体的特点是在宿主细胞外活力迅速下降，有效保存其传染性是后续细胞培养复苏的前提条件。因此，对三株肺炎衣原体储备菌株和三株野生型菌株在模拟运输条件下的细胞外存活情况进行了检测，以便制定临床标本运输和储存的建议。运输培养基中胎牛血清的存在以及冷藏显著提高了长时间运输过程中衣原体的回收率。在伊格尔氏基本培养基或蔗糖 - 磷酸盐 - 谷氨酰胺培养基中，传染性损失保持在最低限度。在22℃储存12小时后，储备菌株的回收率为81%。当冷藏至4℃时，48小时后可实现74%的回收率。尽管这些菌株来自不同地理区域，但良好存活的要求具有可比性，因此应适用于全球。结果表明，实验室菌株并非极其不稳定。然而，对野生型菌株的比较检测显示其稳定性较差：原代分离株在22℃下超过4小时或在4℃下超过24小时后无法令人满意地回收。储存时间的进一步延长导致回收率迅速下降，这表明需要在-75℃下冷冻样品以保持活力。建议遵循原代分离株所获数据表明的较短储存期，以确保成功运输，直至获得更多临床材料的广泛检测结果。
