Montalban G S, Roblin P M, Hammerschlag M R
Department of Pediatrics, SUNY Health Science Center at Brooklyn 11203-2098.
J Clin Microbiol. 1994 May;32(5):1406-7. doi: 10.1128/jcm.32.5.1406-1407.1994.
We evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal reagents and one conjugated genus-specific reagent. All three produced similar intensities of staining of C. pneumoniae, with some strain-to-strain variation. Methanol appeared to be a better choice of fixative than acetone, which greatly reduced the intensity of fluorescence with one of the species-specific antibodies.
我们通过检测三种市售单克隆抗体对八株肺炎衣原体包涵体的染色能力，评估了它们在细胞培养中用于确认肺炎衣原体存在的性能。所测试的抗体为两种未结合的肺炎衣原体特异性单克隆试剂和一种结合的属特异性试剂。所有三种抗体对肺炎衣原体的染色强度相似，但存在一些菌株间差异。甲醇似乎是比丙酮更好的固定剂选择，丙酮会大幅降低其中一种种特异性抗体的荧光强度。
