在无血清培养基中培养可提高肺炎衣原体的分离率。
Growth in serum-free medium improves isolation of Chlamydia pneumoniae.
作者信息
Maass M, Essig A, Marre R, Henkel W
机构信息
Institute of Medical Microbiology, Medical University of Lübeck, Germany.
出版信息
J Clin Microbiol. 1993 Nov;31(11):3050-2. doi: 10.1128/jcm.31.11.3050-3052.1993.
Abstract
Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion counts in a serum-free environment. Serum-free cultivation is recommended to increase the efficiency of C. pneumoniae isolation from clinical material and to permit elementary body purification without interference caused by serum components.
摘要
对在无血清和补充血清的细胞培养基中同时培养的8株肺炎衣原体菌株进行了感染性滴度测定。使用无血清培养基导致HL细胞对衣原体感染的敏感性提高了10至50倍。野生型菌株的比较性初次分离在无血清环境中也产生了更高的包涵体计数。建议采用无血清培养以提高从临床材料中分离肺炎衣原体的效率，并允许在不受血清成分干扰的情况下进行原体纯化。
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