伯氏疏螺旋体外表面蛋白A和C在蓖麻蜱中的表达。
Expression of outer surface proteins A and C of Borrelia burgdorferi in Ixodes ricinus.
作者信息
Fingerle V, Hauser U, Liegl G, Petko B, Preac-Mursic V, Wilske B
机构信息
Max von Pettenkofer Institute für Hygiene und Medizinische Mikrobiologie Universitt München, Germany.
出版信息
J Clin Microbiol. 1995 Jul;33(7):1867-9. doi: 10.1128/jcm.33.7.1867-1869.1995.
Abstract
A total of 472 field-collected Ixodes ricinus ticks from southern Germany were investigated by immunofluorescence for the presence of Borrelia burgdorferi with a polyvalent rabbit immune serum and with monoclonal antibodies specific for outer surface proteins A and C (OspA and OspC, respectively). Borreliae were detected in 90 ticks with the polyvalent immunofluorescence assay. Infection rates in adults (females, 20.2%; males, 25.2%) were significantly higher than in nymphs (12.1%). OspA was detected in 77 ticks and OspC was detected in only 1 tick with the respective monoclonal antibodies. We therefore conclude that B. burgdorferi in unfed I. ricinus ticks usually expresses OspA and very rarely OspC.
摘要
利用多价兔免疫血清以及分别针对外表面蛋白A和C（分别为OspA和OspC）的单克隆抗体，通过免疫荧光法对从德国南部野外采集的472只蓖麻硬蜱进行了调查，以检测伯氏疏螺旋体的存在情况。通过多价免疫荧光检测法在90只蜱中检测到了伯氏疏螺旋体。成虫（雌性为20.2%；雄性为25.2%）的感染率显著高于若虫（12.1%）。利用相应的单克隆抗体，在77只蜱中检测到了OspA，仅在1只蜱中检测到了OspC。因此，我们得出结论，未进食的蓖麻硬蜱中的伯氏疏螺旋体通常表达OspA，很少表达OspC。
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