Barbour A G, Burgdorfer W, Grunwaldt E, Steere A C
J Clin Invest. 1983 Aug;72(2):504-15. doi: 10.1172/jci110998.
Lyme disease is an inflammatory disorder of skin, joints, nervous system, and heart. The disease is associated with a preceding tick bite and is ameliorated by penicillin treatment. A spirochete (IDS) isolated from Ixodes dammini ticks has been implicated as the etiologic agent of Lyme disease. We examined the antibody responses of Lyme disease patients to IDS lysate components in order to further understand the pathogenesis of this disease. The components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, reacted with patients' sera, and the bound IgG was detected with 125I-labeled protein A (western blot). We found that (a) Lyme disease patients had antibodies to IDS components (b) most patients studied had antibodies to two components with apparent subunit molecular weights of 41,000 and 60,000, and (c) the patients' antibody responses during illness and remission were specific, for the most part, for the IDS. In contrast to the findings with Lyme disease sera, sera from controls showed little reactivity with IDS components in either the western blots or a derivative solid-phase radioimmunoassay.
莱姆病是一种皮肤、关节、神经系统和心脏的炎症性疾病。该疾病与先前的蜱虫叮咬有关，青霉素治疗可使其病情改善。从达敏硬蜱中分离出的一种螺旋体（IDS）被认为是莱姆病的病原体。我们检测了莱姆病患者对IDS裂解物成分的抗体反应，以便进一步了解该疾病的发病机制。这些成分通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离，转移至硝酸纤维素膜上，与患者血清反应，然后用125I标记的蛋白A检测结合的IgG（蛋白质印迹法）。我们发现：（a）莱姆病患者对IDS成分有抗体；（b）大多数研究对象的患者对两种成分有抗体，其亚基表观分子量分别为41,000和60,000；（c）患者在患病期间和病情缓解期的抗体反应在很大程度上对IDS具有特异性。与莱姆病血清的检测结果相反，在蛋白质印迹法或衍生的固相放射免疫测定中，对照血清与IDS成分几乎没有反应性。
