Burkot T R, Patrican L, Piesman J
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado.
Am J Trop Med Hyg. 1994 Mar;50(3):354-8. doi: 10.4269/ajtmh.1994.50.354.
Field-collected adult male Ixodes scapularis from Westchester County, New York were bisected and Borrelia burgdorferi infection rates were ascertained by both a direct fluorescent antibody test and an outer surface protein A (OspA) antigen-capture enzyme-linked immunosorbent assay (ELISA). Both assays gave identical antigen positivity rates with 89% concordance between the two assays. Storing dried ticks before ELISA analysis had no significant effect on the ability of the ELISA to determine the presence of OspA compared with assaying live ticks. The OspA antigen positivity rate for dried ticks was 49% compared with 53% for live ticks, with mean OspA antigen spirochete equivalents of 3,388 and 2,823 for dried and live ticks, respectively.
从纽约韦斯特切斯特县野外采集的成年雄性肩突硬蜱被一分为二，通过直接荧光抗体试验和外表面蛋白A（OspA）抗原捕获酶联免疫吸附测定（ELISA）确定伯氏疏螺旋体感染率。两种检测方法的抗原阳性率相同，两种检测方法的一致性为89%。与检测活蜱相比，在ELISA分析前储存干燥蜱对ELISA检测OspA存在的能力没有显著影响。干燥蜱的OspA抗原阳性率为49%，活蜱为53%，干燥蜱和活蜱的平均OspA抗原螺旋体当量分别为3388和2823。
