从临床样本中在人肺胚胎成纤维细胞（MRC - 5）中分离流感病毒。
Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples.
作者信息
de Oña M, Melón S, de la Iglesia P, Hidalgo F, Verdugo A F
机构信息
Microbiology Service, Hospital Central de Asturias (Hospital Covadonga), Oviedo, Spain.
出版信息
J Clin Microbiol. 1995 Jul;33(7):1948-9. doi: 10.1128/jcm.33.7.1948-1949.1995.
Abstract
Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used for this purpose but one frequently used in clinical virology laboratories. Both cell preparations were treated with trypsin. Influenza virus was recovered from 15% of the samples inoculated in MDCK cells and from 18% of those inoculated in MRC-5 cells. The use of MRC-5 cells can simplify the search for respiratory viruses and would assist in the rapid detection of influenza virus during new epidemics.
摘要
对94例疑似流感病毒感染患者的94份咽拭子样本，分别接种于用于分离流感病毒的传统细胞系统——犬肾传代细胞（MDCK），以及为此目的较少使用但在临床病毒学实验室常用的人胚肺成纤维细胞（MRC-5）。两种细胞制剂均用胰蛋白酶处理。在接种于MDCK细胞的样本中，15%分离出流感病毒；在接种于MRC-5细胞的样本中，18%分离出流感病毒。使用MRC-5细胞可简化呼吸道病毒的检测，并有助于在新的疫情期间快速检测流感病毒。
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