Stokes C E, Bernstein J M, Kyger S A, Hayden F G
Research Service, Dayton Veterans Administration Medical Center, Ohio.
J Clin Microbiol. 1988 Jul;26(7):1263-6. doi: 10.1128/jcm.26.7.1263-1266.1988.
Murine monoclonal antibodies directed against type-specific antigens of influenza A and B viruses have been shown to be useful diagnostic reagents for the detection of influenza viruses by immunofluorescence testing of nasopharyngeal cells. We have developed fluorescent focus assays utilizing these antibodies in cell culture chamber slides and shell vials for the rapid diagnosis of influenza A and B. Chamber slide assays were compared with virus isolation in 160 specimens from 135 patients with symptoms of influenza. Virus isolation was compared with immunofluorescence testing in 38 of the 160 specimens. Compared with virus isolation, 24-h cell culture chamber slide assays had a sensitivity of 75% and a specificity of 96%. Immunofluorescence testing of nasopharyngeal cells was only 38% sensitive and 91% specific. Shell vial assays were compared with virus isolation for 89 specimens. At 16 to 18 h postinoculation, the shell vial assay was 84% sensitive and 100% specific. We conclude that both chamber slide and shell vial assays are rapid, sensitive, and specific techniques for the diagnosis of influenza.
针对甲型和乙型流感病毒型特异性抗原的鼠单克隆抗体已被证明是通过对鼻咽细胞进行免疫荧光检测来检测流感病毒的有用诊断试剂。我们利用这些抗体在细胞培养室玻片和微量培养板中开发了荧光灶测定法，用于甲型和乙型流感的快速诊断。将室玻片测定法与135例有流感症状患者的160份标本中的病毒分离法进行了比较。在160份标本中的38份中，将病毒分离法与免疫荧光检测法进行了比较。与病毒分离法相比，24小时细胞培养室玻片测定法的灵敏度为75%，特异性为96%。鼻咽细胞的免疫荧光检测灵敏度仅为38%，特异性为91%。对89份标本的微量培养板测定法与病毒分离法进行了比较。接种后16至18小时，微量培养板测定法的灵敏度为84%，特异性为100%。我们得出结论，室玻片和微量培养板测定法都是诊断流感的快速、灵敏和特异的技术。
