Kohan S A, Pescatori M, Brecha N C, Mastrogiacomo A, Umbach J A, Gundersen C B
Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90024, USA.
J Neurosci. 1995 Sep;15(9):6230-8. doi: 10.1523/JNEUROSCI.15-09-06230.1995.
Cysteine string proteins (csps) are a recently discovered class of cysteine-rich proteins. They have been shown to associate preferentially with synaptic vesicle fractions of Torpedo electric organ or rat brain where they have been implicated in events associated with transmitter secretion. However, to date there has been no information concerning the distribution of csps in rat tissues. We investigated the localization of csps in the rat retina and CNS using immunohistochemistry with affinity purified anti-csp antibodies. Specific csp immunoreactivity having a punctate appearance is present throughout the neuraxis. Csp immunoreactivity is particularly abundant in synapse-rich regions including those of the retina, main olfactory bulb, hippocampal formation, and cerebellum. White matter tracts are devoid of csp immunoreactivity. Neuromuscular junctions show strong csp immunoreactivity. This localization of csp immunoreactivity is compatible with a role for csps in presynaptic events at a wide variety of synapses. Immunohistochemical analysis of a non-neuronal, secretory tissue, the adrenal gland, reveals prominent csp immunoreactivity in the chromaffin cells of the adrenal medulla. However, csp immunoreactivity is not detected in adrenal cortical regions. These findings are confirmed and extended by immunoblot and Northern analyses which identify a 35 kDa and a 5 kb product, respectively, in extracts of adrenal. The presence of csps in the adrenal medulla suggests that these proteins may also participate in secretion-related events in certain non-neuronal cells.
半胱氨酸串珠蛋白（CSPs）是最近发现的一类富含半胱氨酸的蛋白质。已表明它们优先与电鳐电器官或大鼠脑的突触小泡部分相关联，在这些部位它们参与了与递质分泌相关的事件。然而，迄今为止，尚无关于CSPs在大鼠组织中分布的信息。我们使用亲和纯化的抗CSP抗体通过免疫组织化学方法研究了CSPs在大鼠视网膜和中枢神经系统中的定位。在整个神经轴中都存在具有点状外观的特异性CSP免疫反应性。CSP免疫反应性在富含突触的区域特别丰富，包括视网膜、主嗅球、海马结构和小脑的区域。白质束没有CSP免疫反应性。神经肌肉接头显示出强烈的CSP免疫反应性。CSP免疫反应性的这种定位与CSPs在多种突触的突触前事件中的作用相一致。对非神经分泌组织肾上腺的免疫组织化学分析显示，肾上腺髓质的嗜铬细胞中有明显的CSP免疫反应性。然而，在肾上腺皮质区域未检测到CSP免疫反应性。免疫印迹和Northern分析分别在肾上腺提取物中鉴定出35 kDa的产物和5 kb的产物，证实并扩展了这些发现。肾上腺髓质中存在CSPs表明这些蛋白质也可能参与某些非神经细胞中与分泌相关的事件。
