Brink E J, Haddeman E, de Fouw N J, Weststrate J A
Unilever Research Laboratorium, Vlaardingen, The Netherlands.
J Nutr. 1995 Sep;125(9):2379-87. doi: 10.1093/jn/125.9.2379.
In this study the effect of the positional distribution of stearic acid (18:0) and oleic acid (18:1) in a triacylglycerol on absorption of fat, energy and nutrients was investigated in young rats. In addition the effect of dietary calcium on these variables was studied. Forty rats were fed purified diets containing either a fat blend high in 2-oleoyl-distearate or a fat blend high in 1-oleoyl-distearate. Both diets were given at low (0.3 g/100 g) and high (1.0 g/100 g) dietary calcium concentrations. Total fat absorption, expressed as the percentage of fat intake, was significantly lower in rats fed 2-oleoyl-distearate compared with 1-oleoyl-distearate at both dietary calcium concentrations. When expressed as absolute figures, the lower fat absorption in rats fed 2-oleoyl-distearate compared with 1-oleoyl-distearate only reached statistical significance at the high dietary calcium concentration. The reduced absorption of total fat was mainly caused by the lower absorption of stearic acid. The percentage of, but not absolute, absorption of oleic acid and energy were lower in rats fed 2-oleoyl-distearate. Absolute and percentage of calcium absorption were lower in rats fed 2-oleoyl-distearate compared with 1-oleoyl-distearate. Absolute and percentage of magnesium absorption were not significantly affected by the positional distribution of stearic acid and oleic acid in the triacylglycerol, but were decreased at a high dietary calcium concentration. We concluded that the lowered stearic acid absorption from 2-oleoyl-distearate compared with 1-oleoyl-distearate might have been due to the greater formation of insoluble calcium and magnesium soaps in the intestine.
在本研究中，我们调查了三酰甘油中硬脂酸（18:0）和油酸（18:1）的位置分布对幼鼠脂肪、能量及营养物质吸收的影响。此外，还研究了膳食钙对这些变量的影响。40只大鼠被喂食纯化日粮，其中一种日粮含有高比例的2-油酰基-二硬脂酸甘油酯脂肪混合物，另一种日粮含有高比例的1-油酰基-二硬脂酸甘油酯脂肪混合物。两种日粮分别以低（0.3 g/100 g）和高（1.0 g/100 g）膳食钙浓度投喂。在两种膳食钙浓度下，与喂食1-油酰基-二硬脂酸甘油酯的大鼠相比，喂食2-油酰基-二硬脂酸甘油酯的大鼠的总脂肪吸收（以脂肪摄入量的百分比表示）显著降低。以绝对值表示时，喂食2-油酰基-二硬脂酸甘油酯的大鼠与喂食1-油酰基-二硬脂酸甘油酯的大鼠相比，脂肪吸收较低仅在高膳食钙浓度时达到统计学显著差异。总脂肪吸收减少主要是由于硬脂酸吸收较低所致。喂食2-油酰基-二硬脂酸甘油酯的大鼠油酸和能量的吸收百分比（而非绝对值）较低。与喂食1-油酰基-二硬脂酸甘油酯的大鼠相比，喂食2-油酰基-二硬脂酸甘油酯的大鼠钙吸收的绝对值和百分比均较低。三酰甘油中硬脂酸和油酸的位置分布对镁吸收的绝对值和百分比没有显著影响，但在高膳食钙浓度时镁吸收减少。我们得出结论，与1-油酰基-二硬脂酸甘油酯相比，2-油酰基-二硬脂酸甘油酯中硬脂酸吸收降低可能是由于肠道中不溶性钙和镁皂形成增加所致。
