健康对照者和囊性纤维化患者对[1-13C]棕榈酸的胃肠道处理情况。
Gastrointestinal handling of [1-13C]palmitic acid in healthy controls and patients with cystic fibrosis.
作者信息
Murphy J L, Jones A E, Stolinski M, Wootton S A
机构信息
Institute of Human Nutrition, University of Southampton.
出版信息
Arch Dis Child. 1997 May;76(5):425-7. doi: 10.1136/adc.76.5.425.
AIM
To examine the gastrointestinal handling of [1-13C]palmitic acid given as the free acid by measuring the excretion of 13C label in stool in 16 healthy children and 11 patients with cystic fibrosis on their habitual enzyme replacement treatment.
METHODS
After an overnight fast, each child ingested 10 mg/kg body weight [1-13C]palmitic acid with a standardised test meal of low natural 13C abundance. A stool sample was collected before the test and all stools were collected thereafter for a period of up to five days. The total enrichment of 13C in stool and the species bearing the 13C label was measured using isotope ratio mass spectrometry.
RESULTS
The proportion of administered 13C label excreted in stool was 24.0% (range 10.7-64.9%) in healthy children and only 4.4% (range 1.2-11.6%) in cystic fibrosis patients. The enrichment of 13C in stool was primarily restricted to the species consumed by the subjects (that is as palmitic acid).
CONCLUSIONS
There does not appear to be a specific defect in the absorption of [1-13C]palmitic acid in patients with cystic fibrosis. The reasons why cystic fibrosis patients appear to absorb more of this saturated fatty acid than healthy children is not clear and requires further investigation.
目的
通过测量16名健康儿童和11名接受常规酶替代治疗的囊性纤维化患者粪便中13C标记物的排泄情况，研究以游离酸形式给予的[1-13C]棕榈酸在胃肠道中的处理过程。
方法
禁食过夜后，每个儿童摄入10mg/kg体重的[1-13C]棕榈酸，并搭配天然13C丰度较低的标准化测试餐。在测试前收集一份粪便样本，此后收集所有粪便，最长持续五天。使用同位素比率质谱法测量粪便中13C的总富集量以及带有13C标记的物质。
结果
健康儿童粪便中排泄的给予的13C标记物比例为24.0%（范围10.7 - 64.9%），而囊性纤维化患者仅为4.4%（范围1.2 - 11.6%）。粪便中13C的富集主要局限于受试者摄入的物质（即作为棕榈酸）。
结论
囊性纤维化患者在[1-13C]棕榈酸的吸收方面似乎不存在特定缺陷。囊性纤维化患者比健康儿童吸收更多这种饱和脂肪酸的原因尚不清楚，需要进一步研究。
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