通过E3L突变体拯救缺失E3L基因的痘苗病毒。
Rescue of vaccinia virus lacking the E3L gene by mutants of E3L.
作者信息
Chang H W, Uribe L H, Jacobs B L
机构信息
Department of Microbiology, Arizona State University, Tempe 85287-2701, USA.
出版信息
J Virol. 1995 Oct;69(10):6605-8. doi: 10.1128/JVI.69.10.6605-6608.1995.
Abstract
Vaccinia virus with the E3L gene deleted was able to replicate in RK-13 but not HeLa cells. This host range phenotype could be complemented by an E3L gene expressed transiently from a plasmid. Analysis of mutants of E3L indicates that the ability to complement deletion of E3L correlates with the ability of mutated proteins to bind double-stranded RNA but not with their ability to migrate to the nucleus.
摘要
缺失E3L基因的痘苗病毒能够在RK - 13细胞中复制，但不能在HeLa细胞中复制。这种宿主范围表型可以通过从质粒瞬时表达的E3L基因来互补。对E3L突变体的分析表明，互补E3L缺失的能力与突变蛋白结合双链RNA的能力相关，而与其迁移到细胞核的能力无关。
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