Miyamura K, Sadahiro S, Konda T, Takahashi M, Fujino R, Nishimura Y, Miyakoshi H, Horiuchi K, Furuya Y, Kubota T
Department of Epidemiology, National Institute of Health, Tokyo.
Jpn J Med Sci Biol. 1995 Feb;48(1):49-59. doi: 10.7883/yoken1952.48.49.
The gelatin-particle-agglutination (PA) test for titrating antibodies against diphtheria, pertussis and tetanus toxins was developed and used for assaying 65 sera from healthy children to assess the antitoxin acquisition in relation to the administration of adsorbed diphtheria-purified pertussis-tetanus (DPT) combined vaccine. The antitoxin titers obtained by the PA test and the conventional methods were correlated well; the correlation coefficient of the diphtheria antitoxin titers between the PA test and the cell culture method was 0.908, that of the tetanus antitoxin titers between the PA test and the passive hemagglutination test 0.968, and that of anti-pertussis toxin titers between the PA test and polystyrene-ball ELISA 0.885. The PA test was shown to be useful in both developed and developing countries, since it is simple to perform, sensitive and specific, and the three antitoxins can be titrated by the same procedure.
开发了用于滴定抗白喉、百日咳和破伤风毒素抗体的明胶颗粒凝集（PA）试验，并用于检测65名健康儿童的血清，以评估与吸附白喉-纯化百日咳-破伤风（DPT）联合疫苗接种相关的抗毒素获得情况。通过PA试验和传统方法获得的抗毒素滴度相关性良好；PA试验与细胞培养法之间白喉抗毒素滴度的相关系数为0.908，PA试验与被动血凝试验之间破伤风抗毒素滴度的相关系数为0.968，PA试验与聚苯乙烯球ELISA之间抗百日咳毒素滴度的相关系数为0.885。PA试验在发达国家和发展中国家均显示出有用性，因为它操作简单、灵敏且特异，并且三种抗毒素可通过相同程序进行滴定。
