乙醇对因戊巴比妥睡眠时间反应不同而被挑选出的小鼠的低温效应。

Hypothermic effect of ethanol in mice selected for differential sleep-time response to pentobarbital.

作者信息

Mizinga K M, Stino F K, Samaan S S, Soliman K F, Kolta M G

机构信息

College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee 32307, USA.

出版信息

Pharmacol Biochem Behav. 1995 Jun-Jul;51(2-3):525-8. doi: 10.1016/0091-3057(95)00049-3.

DOI:10.1016/0091-3057(95)00049-3

PMID:7667380

Abstract

The hypothermic action of ethanol was investigated in genetically distinct lines of mice selected for sleep-time response to pentobarbital for six generations. Ethanol (3 g/kg, intraperitoneally) was administered to alcohol-naive males and females from each of the unselected control, long-, and short-sleep mouse lines. Rectal temperatures were measured immediately before, and at 15, 30, 60, 90, 120, and 240 min after ethanol injection. Eight female and eight male mice from each line were sacrificed at each time point, and trunk blood was collected for plasma ethanol analysis. The results show that short-sleep mice were less hypothermic (p < 0.05) compared to long-sleep mice at 15 and 30 min after ethanol administration. However, plasma ethanol concentrations were not significantly different between the mouse lines at any time point. Therefore, the line-dependent differential ethanol-induced hypothermia observed may be a result of differences in "brain sensitivity" rather than in the rates of ethanol metabolism among the mouse lines.

摘要

在经过六代对戊巴比妥睡眠时间反应筛选的基因不同品系小鼠中，研究了乙醇的低温作用。对来自未选对照组、长睡眠和短睡眠小鼠品系的未接触过酒精的雄性和雌性小鼠腹腔注射乙醇（3克/千克）。在乙醇注射前以及注射后15、30、60、90、120和240分钟测量直肠温度。每个品系在每个时间点处死8只雌性和8只雄性小鼠，并采集躯干血进行血浆乙醇分析。结果显示，在乙醇给药后15和30分钟时，与长睡眠小鼠相比，短睡眠小鼠的体温降低幅度较小（p<0.05）。然而，在任何时间点，小鼠品系之间的血浆乙醇浓度均无显著差异。因此，观察到的品系依赖性乙醇诱导的低温差异可能是小鼠品系之间“脑敏感性”差异而非乙醇代谢速率差异的结果。