Liu Y S, Du W P, Chen M, Wu Z X
Department of Parasitology, Xuzhou Medical College, Jiangsu, China.
Southeast Asian J Trop Med Public Health. 1994 Dec;25(4):724-7.
Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1:184 (1:10-1:2560) for Dot-IGSS and 1:150 (1:10-1:2560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.
采用马来布鲁线虫可溶性抗原，运用斑点免疫金-银染色法（Dot-IGSS）和斑点酶联免疫吸附测定法（Dot-ELISA）检测50例班氏吴策线虫微丝蚴血症患者血清中的抗班氏吴策线虫抗体。Dot-IGSS和Dot-ELISA的阳性率分别为100%和90%。45例阳性患者中，Dot-IGSS法检测抗体平均效价为1:184（1:10 - 1:2560），Dot-ELISA法为1:150（1:10 - 1:2560）。两种方法检测结果中，30例效价相同，13例Dot-IGSS法效价高于Dot-ELISA法，2例Dot-IGSS法效价低于Dot-ELISA法。Dot-IGSS法与Dot-ELISA法检测抗体效价呈线性关系（r = 0.8443）。Dot-IGSS法与Dot-ELISA法一样，操作简便，结果易判读。可见，Dot-IGSS法具有高度敏感性和特异性，可用于丝虫病的免疫诊断及监测。
