[三种血清学检测方法用于检测班氏丝虫微丝蚴血症患者抗体的比较研究]

[Comparative study on three serologic tests for detecting antibodies in cases with bancroftian microfilaremia].

作者信息

Sun D, Yuan Y, Shi H, Luo M, Chen S, Shi Z

机构信息

Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(3):185-7.

PMID:7867152

Abstract

A comparative study was conducted for detecting antibody levels of bancroftian microfilaremia cases by using three serologic tests, IFAT, ELISA and dot-ELISA. A total of 102 serum specimens were collected from bancroftian microfilaremia cases. The positive rate was 89.2% (91/102) with IFAT (serum dilution > or = 1:20), 84.3% (86/102) with ELISA (OD value > or = 0.440) and 89.2% (91/102) with dot-ELISA (serum dilution > or = 1:80), respectively (P > 0.05); while in healthy individuals from non-endemic areas the false positive rate was 0(0/30), 1.8(1/55) and 0(0/54), respectively. The results indicated that there were no statistically significant differences among the three serologic tests. There was no correlation found in this study between microfilaria density and the antibody level. It was also proved that the detection rate of the combined use of either 2 or 3 tests was higher than each of the three tests; the positive rates being 94.1%-98.0%.

摘要

采用间接荧光抗体试验（IFAT）、酶联免疫吸附试验（ELISA）和斑点酶联免疫吸附试验（dot-ELISA）三种血清学检测方法，对班氏丝虫微丝蚴血症患者的抗体水平进行了比较研究。共收集了102例班氏丝虫微丝蚴血症患者的血清标本。IFAT（血清稀释度≥1:20）的阳性率为89.2%（91/102），ELISA（OD值≥0.440）的阳性率为84.3%（86/102），dot-ELISA（血清稀释度≥1:80）的阳性率为89.2%（91/102），三者差异无统计学意义（P>0.05）；而在非流行区健康人群中，IFAT、ELISA和dot-ELISA的假阳性率分别为0（0/30）、1.8%（1/55）和0（0/54）。结果表明，三种血清学检测方法之间差异无统计学意义。本研究未发现微丝蚴密度与抗体水平之间存在相关性。同时也证明，联合使用2种或3种检测方法的检出率高于单独使用三种检测方法中的任何一种，阳性率为94.1% - 98.0%。