Griga T, Tromm A, Schwegler U, May B
Department of Gastroenterology and Hepatology, University Hospital Bergmannsheil, Bochum, Germany.
Z Gastroenterol. 1995 Jul;33(6):345-8.
During active inflammatory bowel disease (IBD) the respiratory burst of neutrophil granulocytes has been shown to be impaired using isolated circulating neutrophil granulocytes of patients with active disease. Using normal neutrophil granulocytes of healthy volunteers the present study examines the potential priming effect of sera of patients with active and quiescent IBD. The superoxide anion (O2-)-release of normal neutrophil granulocytes in response to N-formyl-methionyl-leucyl-phenylalanine (FMLP) has been investigated after incubation with sera of patients with active and inactive Crohn's disease and ulcerative colitis. O2(-)-release was measured using the superoxide dismutase inhibitable reduction of ferricytochrome c. The O2(-)-release of normal neutrophil granulocytes primed with sera of patients with inactive Crohn's disease (607.1 +/- 218.2 nmol/60 min, n = 10, p = 0.001) or cultured with sera of patients with quiescent ulcerative colitis (497.4 +/- 94.9 nmol/60 min, n = 3, p = 0.005) was significantly enhanced when compared with sera of normal controls (319.8 +/- 86.5 nmol/60 min, n = 10). There was no significant difference between priming with sera of patients with quiescent or active Crohn's disease (481.0 +/- 113.0 nmol/60 min, n = 5). Normal neutrophil granulocytes primed with sera of patients with active ulcerative colitis produce significantly larger amounts of O2- (809.5 +/- 256.9 nmol/60 min, n = 4, p = 0.001) when compared with sera of normal controls. The study shows that sera of patients with quiescent IBD as well as sera of patients with active disease have the potential to prime normal neutrophil granulocytes for an enhanced O2(-)-release in response to FMLP.(ABSTRACT TRUNCATED AT 250 WORDS)
在活动性炎症性肠病（IBD）期间，使用活动期疾病患者的循环中性粒细胞进行分离检测，结果显示中性粒细胞的呼吸爆发受损。本研究利用健康志愿者的正常中性粒细胞，检测活动期和静止期IBD患者血清的潜在启动作用。在分别与活动期和静止期克罗恩病及溃疡性结肠炎患者的血清孵育后，研究了正常中性粒细胞对N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）反应时超氧阴离子（O2-）的释放情况。采用超氧化物歧化酶抑制的细胞色素c还原法测定O2-释放量。与正常对照血清（319.8±86.5 nmol/60分钟，n = 10）相比，用静止期克罗恩病患者血清启动的正常中性粒细胞O2-释放量（607.1±218.2 nmol/60分钟，n = 10，p = 0.001）或与静止期溃疡性结肠炎患者血清共同培养的正常中性粒细胞O2-释放量（497.4±94.9 nmol/60分钟，n = 3，p = 0.005）显著增加。静止期或活动期克罗恩病患者血清启动作用之间无显著差异（481.0±113.0 nmol/60分钟，n = 5）。与正常对照血清相比，用活动期溃疡性结肠炎患者血清启动的正常中性粒细胞产生的O2-量显著更大（809.5±256.9 nmol/60分钟，n = 4，p = 0.001）。该研究表明，静止期IBD患者的血清以及活动期疾病患者的血清均有可能启动正常中性粒细胞，使其对FMLP反应时增强O2-释放。（摘要截选至250词）
