Nikolaus S, Bauditz J, Gionchetti P, Witt C, Lochs H, Schreiber S
Charité University Hospital, 4th Medical Department, Humboldt University, Berlin, Germany.
Gut. 1998 Apr;42(4):470-6. doi: 10.1136/gut.42.4.470.
Concentrations of pro-inflammatory cytokines are increased in the intestinal mucosa of patients with active inflammatory bowel disease (IBD). Polymorphonuclear neutrophil granulocytes (PMN) are the most abundant cell type in intestinal lesions in IBD. Interleukin 10 (IL-10) is an important contra-inflammatory cytokine which induces downregulation of pro-inflammatory cytokines.
To investigate whether PMN from patients with IBD or infectious colitis, respectively, secrete increased amounts of pro-inflammatory cytokines and can be regulated by IL-10.
Secretion (ELISA) as well as corresponding mRNA levels (semiquantitative RT-PCR) of pro-inflammatory cytokines (IL-1 beta, TNF-alpha) and of IL-1 receptor antagonist were assessed in peripheral PMN.
PMN from patients with IBD are primed to secrete enhanced amounts of pro-inflammatory cytokines accompanied by detection of corresponding mRNAs in comparison with normal controls. This finding is not specific for IBD but rather reflects intestinal inflammation in general. IL-10 markedly inhibited proinflammatory cytokine secretion as well as corresponding mRNA concentrations.
PMN are an important source of pro-inflammatory cytokines in patients with intestinal inflammation and can be downregulated by IL-10.
在活动性炎症性肠病(IBD)患者的肠道黏膜中,促炎细胞因子的浓度会升高。多形核中性粒细胞(PMN)是IBD肠道病变中最丰富的细胞类型。白细胞介素10(IL-10)是一种重要的抗炎细胞因子,可诱导促炎细胞因子的下调。
分别研究IBD患者或感染性结肠炎患者的PMN是否分泌更多的促炎细胞因子,以及是否可被IL-10调节。
通过酶联免疫吸附测定法(ELISA)以及半定量逆转录聚合酶链反应(RT-PCR)评估外周血PMN中促炎细胞因子(IL-1β、TNF-α)和IL-1受体拮抗剂的分泌情况及其相应的mRNA水平。
与正常对照相比,IBD患者的PMN易于分泌更多的促炎细胞因子,同时可检测到相应的mRNA。这一发现并非IBD所特有,而是反映了一般性的肠道炎症。IL-10可显著抑制促炎细胞因子的分泌以及相应的mRNA浓度。
PMN是肠道炎症患者促炎细胞因子的重要来源,且可被IL-10下调。