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用人横纹肌肉瘤细胞系(HUS-2)的无细胞提取物对人胚胎细胞进行转化:简报

Transformation of human embryo cells with the use of cell-free extracts of a human rhabdomyosarcoma cell line (HUS-2): brief communication.

作者信息

Cook B, O'Sullivan F, Leung J, Morse P, Graham B, Chapman A L

出版信息

J Natl Cancer Inst. 1978 May;60(5):979-84. doi: 10.1093/jnci/60.5.979.

Abstract

Cell-free extracts of the human rhabdomyosarcoma cell line HUS-2 caused the transformation of human embryo fibroblasts. This transformation included morphologic alteration, karyotypic change, and an increase in culture longevity. With the use of sex markers, multiple karyotypes confirmed that the human embryo fibroblasts were transformed, and the use of cell-free material further suggested the presence of a transforming virus. RNA-dependent DNA polymerase activity in a particle with a specific gravity of 1.16 g/cm3 indicated the presence of an RNA type C virus. Evidence also suggested that the known mammalian type C viruses, routine cytopathic effect-inducing viruses, or mycoplasma were not the agents responsible for the transformation. That both the donor (HUS-2) and converted (HUE-T) cell lines cross-reacted with antisera prepared against HUE-T indicated a common antigen arising in the process of conversion of HUS-2 cells to HUE-T cells.

摘要

人横纹肌肉瘤细胞系HUS-2的无细胞提取物可导致人胚胎成纤维细胞发生转化。这种转化包括形态学改变、核型变化以及培养寿命的延长。利用性标记物,多种核型证实人胚胎成纤维细胞发生了转化,并且使用无细胞物质进一步提示存在一种转化病毒。比重为1.16 g/cm³ 的颗粒中的RNA依赖性DNA聚合酶活性表明存在一种C型RNA病毒。有证据还表明,已知的哺乳动物C型病毒、常规致细胞病变效应病毒或支原体并非导致转化的病原体。供体细胞系(HUS-2)和转化后的细胞系(HUE-T)均与针对HUE-T制备的抗血清发生交叉反应,这表明在HUS-2细胞转化为HUE-T细胞的过程中产生了一种共同抗原。

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