Bhopale K K, Pradhan K S, Masani K B, Kaul C L
Parasitology Department, Boots Pharmaceuticals Ltd., Bombay, India.
Ann Trop Med Parasitol. 1995 Jun;89(3):253-9. doi: 10.1080/00034983.1995.11812950.
Caecal amoebiasis models (mouse, hamster and rat) were compared for amoebic infection and chemotherapeutic evaluation using a pathogenic strain of Entamoeba histolytica of human origin. Caecal infection in the hamster was of the acute type, with a large number of amoebae in the caecal tissue, whereas the amoebae in rats were seen in the lumen, on the mucosal surface. Amoebic lesions in the mouse caecum were of moderate severity. The mouse model responded to both metronidazole (tissue amoebicide) and diloxanide furoate (luminal amoebicide), whereas the hamster model only responded well to metronidazole and the rat model only to diloxanide furoate. Using the mouse as a primary screening model, a new oxadiazole compound, BTI 2405E, was found to have tissue and luminal amoebicidal activity. In rodent caecal models, a single dose of BTI 2405E was found to be more effective than one of metronidazole or diloxanide furoate. The mouse model appears to be the most useful for primary screening of anti-amoebic compounds.
使用源自人类的致病性溶组织内阿米巴菌株，对盲肠阿米巴病模型（小鼠、仓鼠和大鼠）进行了阿米巴感染和化疗评估比较。仓鼠的盲肠感染为急性类型，盲肠组织中有大量阿米巴，而大鼠的阿米巴出现在肠腔和黏膜表面。小鼠盲肠的阿米巴病变程度中等。小鼠模型对甲硝唑（组织杀阿米巴药）和糠酸二氯尼特（肠腔杀阿米巴药）均有反应，而仓鼠模型仅对甲硝唑反应良好，大鼠模型仅对糠酸二氯尼特反应良好。以小鼠作为主要筛选模型，发现一种新的恶二唑化合物BTI 2405E具有组织和肠腔杀阿米巴活性。在啮齿动物盲肠模型中，发现单剂量的BTI 2405E比甲硝唑或糠酸二氯尼特中的一种更有效。小鼠模型似乎是抗阿米巴化合物初步筛选中最有用的模型。
