来自葡酒毛霉的α-半乳糖苷酶cDNA的核苷酸序列。

Nucleotide sequence of alpha-galactosidase cDNA from Mortierella vinacea.

作者信息

Shibuya H, Kobayashi H, Kasamo K, Kusakabe I

机构信息

Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

出版信息

Biosci Biotechnol Biochem. 1995 Jul;59(7):1345-8. doi: 10.1271/bbb.59.1345.

DOI:10.1271/bbb.59.1345

PMID:7670198

Abstract

To analyze the primary structure of Mortierella vinacea alpha-galactosidase, a cDNA library of M. vinacea mRNA in lambda gt10 was constructed. A clone, which has an insert size of about 1.4 kilobase pairs, was found to contain the coding region of the mature enzyme. The deduced amino acid sequence share that the mature enzyme consisted of 397 amino acid residues with a molecular mass of 44,350 Da. The sequence identity of the mature enzyme with alpha-galactosidases from Saccharomyces carlsbergensis, Cyamopsis tetragonoloba (guar), and human were 47%, 43%, and 34%, respectively.

摘要

为分析葡酒毛霉α-半乳糖苷酶的一级结构，构建了λgt10载体中的葡酒毛霉mRNA cDNA文库。发现一个插入片段大小约为1.4千碱基对的克隆包含成熟酶的编码区。推导的氨基酸序列表明成熟酶由397个氨基酸残基组成，分子量为44350道尔顿。该成熟酶与卡尔斯伯酵母、瓜尔豆和人类的α-半乳糖苷酶的序列同一性分别为47%、43%和34%。