Monda M, Amaro S, Sullo A, De Luca B
Department of Human Physiology and Integrated Biological Function, Filippo Bottazzi, Second University of Naples, Italy.
J Physiol Paris. 1994;88(6):347-52. doi: 10.1016/0928-4257(94)90027-2.
The experiment described here tests the effect of intracerebroventricular (icv) injection of nitric oxide (NO) precursors, such as L-arginine (L-arg) and nitroprusside (NP), on the thermogenic changes induced by lesion of the lateral hypothalamus (LH). The firing rate of the nerves innervating interscapular brown adipose tissue (IBAT), along with IBAT and colonic temperatures (TIBAT and TC) were monitored in urethane-anaesthetized male Sprague-Dawley rats lesioned in the LH. These variables were measured before and after an icv injection of 4 mumol L-arg or 400 nmol NP. The same variables were also monitored in: a) lesioned rats with icv administration of saline; b) sham-lesioned animals with icv injection of L-arg or NP; c) sham-lesioned rats with icv injection of saline. The results show that L-arg or NP injection reduces the increases in firing rate. TIBAT and TC induced by LH lesion. These findings suggest that NO plays a key role in the thermogenic changes following LH lesion.
本文所述实验检测了脑室内（icv）注射一氧化氮（NO）前体，如L-精氨酸（L-arg）和硝普钠（NP），对下丘脑外侧区（LH）损伤所致产热变化的影响。在氨基甲酸乙酯麻醉的、LH损伤的雄性Sprague-Dawley大鼠中，监测支配肩胛间棕色脂肪组织（IBAT）的神经放电频率，以及IBAT和结肠温度（TIBAT和TC）。在icv注射4 μmol L-arg或400 nmol NP之前和之后测量这些变量。还在以下大鼠中监测了相同变量：a）icv注射生理盐水的损伤大鼠；b）icv注射L-arg或NP的假损伤动物；c）icv注射生理盐水的假损伤大鼠。结果表明，L-arg或NP注射可降低LH损伤所致的放电频率、TIBAT和TC的升高。这些发现提示，NO在LH损伤后的产热变化中起关键作用。
