Berry R, Stevens T J, Walter N A, Wilcox A S, Rubano T, Hopkins J A, Weber J, Goold R, Soares M B, Sikela J M
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262, USA.
Nat Genet. 1995 Aug;10(4):415-23. doi: 10.1038/ng0895-415.
Using our data set of 3,143 single pass sequences from human brain cDNA libraries, we have developed a strategy in which gene-based sequence-tagged-sites (STSs), derived from 3'untranslated regions of human cDNAs, are rapidly assigned to megabase-insert yeast artificial chromosomes and somatic cell hybrids to generate regional gene mapping data. Employing this approach, we have mapped 318 cDNAs, representing 308 human genes. Ninety-two of these mapped to regions implicated in human genetic diseases, identifying them as candidate genes. Extension of this strategy has the potential to result in virtually every human gene having, at its 3' end, its own associated STS, with each STS in turn specifying both a corresponding genomic clone and a specific regional location in the genome.
利用我们从人脑cDNA文库中获得的3143个单通道序列数据集，我们开发了一种策略，其中源自人类cDNA 3'非翻译区的基于基因的序列标签位点（STS）被快速定位到百万碱基插入酵母人工染色体和体细胞杂种上，以生成区域基因图谱数据。采用这种方法，我们已经定位了318个cDNA，代表308个人类基因。其中92个定位到与人类遗传疾病相关的区域，将它们鉴定为候选基因。扩展这一策略有可能使几乎每个人类基因在其3'端都有自己相关的STS，每个STS又依次指定一个相应的基因组克隆和基因组中的一个特定区域位置。
