Freeman F M, Rose S P, Scholey A B
Brain and Behaviour Research Group, Open University, Milton Keynes, UK.
Neurobiol Learn Mem. 1995 May;63(3):291-5. doi: 10.1006/nlme.1995.1034.
The antibiotic anisomycin (ANI), a protein synthesis inhibitor, was used to investigate the time-related changes in protein synthesis following passive avoidance training in the day-old chick. Retention of memory for this simple learning task is known to be prevented by protein synthesis inhibitors within the first hour post-training. Here we report a second, later time window during which inhibition of protein synthesis results in amnesia following one-trial passive avoidance training. Birds were given bilateral intracranial injections of ANI (10 microliters/hemisphere of a 30 mM solution) at various times relative to training and tested 24 h later. Injections given between 0.5 h prior to 1.5 h post-training or 4-5 h post-training, but not at later or at intervening times, resulted in amnesia. These results are discussed in the context of earlier findings, using the inhibitor of glycoprotein synthesis 2-deoxygalactose, that memory formation shows two glycoprotein-synthesis-dependent periods of sensitivity (Scholey, Rose, Zamani, Bock, & Schachner, 1993). The time windows of susceptibility of ANI and 2-Dgal are consistent with a model in which there are two waves of neural activity following training; during the second, commencing 4 h after training, proteins are synthesized and then glycosylated as part of the establishment of an enduring memory trace.
抗生素茴香霉素（ANI）是一种蛋白质合成抑制剂，被用于研究一日龄雏鸡被动回避训练后蛋白质合成随时间的变化。已知在训练后的第一小时内，蛋白质合成抑制剂会阻止对这项简单学习任务的记忆保留。在此我们报告第二个较晚的时间窗口，在一次被动回避训练后，此期间蛋白质合成受到抑制会导致失忆。在相对于训练的不同时间给鸟类双侧脑内注射ANI（30 mM溶液，10微升/半球），并在24小时后进行测试。在训练前0.5小时至训练后1.5小时之间或训练后4 - 5小时注射，但在更晚或中间时间注射则不会，会导致失忆。这些结果结合早期使用糖蛋白合成抑制剂2 - 脱氧半乳糖的研究结果进行了讨论，即记忆形成显示出两个依赖糖蛋白合成的敏感时期（肖利、罗斯、扎马尼、博克和沙赫纳，1993年）。ANI和2 - Dgal的敏感时间窗口与一个模型一致，即训练后有两波神经活动；在第二波，从训练后4小时开始，蛋白质被合成，然后进行糖基化，作为建立持久记忆痕迹的一部分。
