Chadha H S, Wooley P H, Sud S, Fitzgerald R H
Department of Orthopaedic Surgery, Wayne State University School of Medicine, Hutzel Hospital, Detroit, MI 48201, USA.
Inflamm Res. 1995 Apr;44(4):145-51. doi: 10.1007/BF01782811.
The in vitro response of peripheral blood mononuclear cells (MNC) from patients with cemented total hip or knee arthroplasties, and control individuals, to polymethylmethacrylate (PMMA) was assessed by cell proliferation, cytokine production, and molecular techniques. After seven days in culture, a dose-dependent proliferative response to PMMA stimulation was observed in MNC from fifteen normal individuals. A concomitant dose-dependent production of both IL-1 beta and IL-2 in response to PMMA stimulation was detected. Reverse transcription-polymerase chain reactions (RT-PCR) indicated that both IL-1 beta and IL-2 mRNA was present after 48 hours in culture with PMMA. Cellular proliferation and cytokine production (both IL-1 beta and IL-2) in 10 patients with stable, painless, well-functioning, cemented arthroplasties was significantly lower (p < 0.025) than normal controls and patients with aseptically loosened, painful, arthroplasties. The findings suggest that patients with stable cemented total joint arthroplasties are either inherently or adaptively less responsive to PMMA at a cellular level.
通过细胞增殖、细胞因子产生及分子技术，评估了骨水泥型全髋关节或膝关节置换术患者及对照个体的外周血单个核细胞（MNC）对聚甲基丙烯酸甲酯（PMMA）的体外反应。培养7天后，在15名正常个体的MNC中观察到对PMMA刺激的剂量依赖性增殖反应。检测到伴随PMMA刺激的IL-1β和IL-2的剂量依赖性产生。逆转录-聚合酶链反应（RT-PCR）表明，在与PMMA共培养48小时后，IL-1β和IL-2 mRNA均存在。10例骨水泥型置换术稳定、无痛、功能良好的患者的细胞增殖及细胞因子产生（IL-1β和IL-2）显著低于正常对照及无菌性松动、疼痛的置换术患者（p<0.025）。这些发现提示，骨水泥型全关节置换术稳定的患者在细胞水平上对PMMA的反应要么天生较低，要么适应性较差。
