人巨细胞病毒导致单核细胞中Vβ12 T细胞内HIV-1复制增强：一种假定的疱疹病毒超抗原的证据。

Enhanced HIV-1 replication in V beta 12 T cells due to human cytomegalovirus in monocytes: evidence for a putative herpesvirus superantigen.

作者信息

Dobrescu D, Ursea B, Pope M, Asch A S, Posnett D N

机构信息

Department of Medicine, Graduate School of Medical Sciences, Cornell University Medical College, New York, New York 10021, USA.

出版信息

Cell. 1995 Sep 8;82(5):753-63. doi: 10.1016/0092-8674(95)90472-7.

DOI:10.1016/0092-8674(95)90472-7

PMID:7671303

Abstract

HIV-1 replicates more efficiently in cultured IL-2-dependent CD4 T cells expressing V beta 12 T cell receptors (TCRs) rather than other TCRs (Laurence et al., 1992). A viral reservoir is frequently established in V beta 12 T cells in HIV-1-infected patients. Here we show that cytomegalovirus (CMV) is responsible for V beta 12-selective HIV-1 replication that is indistinguishable from the effect of known superantigens (SAGs). This effect is dependent on direct contact of T cells with CMV-infected monocytes. CMV infection, but not ie1 or ie2 transfection, reproduces this effect in a monocytoid cell line (U937). In HIV-infected patients, the presence of CMV antibodies correlates with an HIV-1 viral load preferentially skewed to the V beta 12 subset. Together, these data suggest that a CMV gene product is responsible for a SAG-driven V beta 12-selective HIV-1 reservoir in vivo.

摘要

HIV-1在表达Vβ12 T细胞受体（TCR）的培养的白细胞介素-2依赖性CD4 T细胞中比在其他TCR中更有效地复制（劳伦斯等人，1992年）。在HIV-1感染患者的Vβ12 T细胞中经常建立病毒储存库。在这里，我们表明巨细胞病毒（CMV）是Vβ12选择性HIV-1复制的原因，这种复制与已知超抗原（SAG）的作用无法区分。这种作用取决于T细胞与CMV感染的单核细胞的直接接触。CMV感染而非ie1或ie2转染在单核细胞样细胞系（U937）中重现了这种作用。在HIV感染患者中，CMV抗体的存在与优先偏向Vβ12亚群的HIV-1病毒载量相关。总之，这些数据表明CMV基因产物是体内SAG驱动的Vβ12选择性HIV-1储存库的原因。