Currie D A, Bate M
Department of Zoology, University of Washington, Seattle 98195, USA.
Development. 1995 Aug;121(8):2549-57. doi: 10.1242/dev.121.8.2549.
The adult abdominal muscles in Drosophila are generated de novo during metamorphosis and form a simple and characteristic pattern. Throughout adult abdominal development there is a close association between nerves and myoblasts. However, the role of innervation in adult myogenesis is unclear. In males there is an additional muscle, which is unique to abdominal segment 5 (A5). This male specific muscle forms from the same pool of myoblasts as other dorsal muscles in A5 but develops several distinctive characteristics. Previous work indicates the genotype of the innervation of this male specific muscle may play a crucial role in its proper development, although the part played by innervation in the development of other muscles is unknown. Here we test directly the function of innervation in adult myogenesis in general and for the development and differentiation of the male specific muscle in particular. After denervation at the onset of metamorphosis, muscle growth is impaired although the overall muscle pattern continues to develop. Uniquely, the male specific muscle fails to form. Our results indicate that there is an essential role for innervation during the period of metamorphosis for the formation of a full complement of abdominal muscles and for muscle growth. Furthermore, innervation is absolutely required for the formation of the male specific muscle and the development of its special characteristics.
果蝇的成虫腹部肌肉在变态发育过程中重新生成，并形成一种简单且具有特征性的模式。在整个成虫腹部发育过程中，神经与成肌细胞之间存在密切联系。然而，神经支配在成虫肌生成中的作用尚不清楚。在雄性果蝇中，存在一块额外的肌肉，它是腹部第5节（A5）所特有的。这块雄性特异性肌肉与A5中其他背侧肌肉来源于同一组成肌细胞池，但具有几个独特的特征。先前的研究表明，这块雄性特异性肌肉的神经支配基因型可能在其正常发育中起关键作用，尽管神经支配在其他肌肉发育中所起的作用尚不清楚。在这里，我们直接测试了神经支配在一般成虫肌生成中以及特别是在雄性特异性肌肉的发育和分化中的功能。在变态发育开始时进行去神经支配后，肌肉生长受到损害，尽管整体肌肉模式仍在继续发育。独特的是，雄性特异性肌肉未能形成。我们的结果表明，在变态发育期间，神经支配对于形成完整的腹部肌肉补充和肌肉生长起着至关重要的作用。此外，神经支配对于雄性特异性肌肉的形成及其特殊特征的发育是绝对必需的。
