Broadie K, Bate M
Department of Zoology, University of Cambridge, UK.
Development. 1993 Oct;119(2):533-43. doi: 10.1242/dev.119.2.533.
We have examined the role of innervation in directing embryonic myogenesis, using a mutant (prospero), which delays the pioneering of peripheral motor nerves of the Drosophila embryo. In the absence of motor nerves, myoblasts fuse normally to form syncytial myotubes, myotubes form normal attachments to the epidermis, and a larval musculature comparable to the wild-type pattern is generated and maintained. Likewise, the twist-expressing myoblasts that prefigure the adult musculature segregate normally in the absence of motor nerves, migrate to their final embryonic positions and continue to express twist until the end of embryonic development. In the absence of motor nerves, myotubes uncouple at the correct developmental stage to form single cells. Subsequently, uninnervated myotubes develop the mature electrical and contractile properties of larval muscles with a time course indistinguishable from normally innervated myotubes. We conclude that innervation plays no role in the patterning, morphogenesis, maintenance or physiological development of the somatic muscles in the Drosophila embryo.
我们利用一种突变体（prospero）研究了神经支配在指导胚胎肌生成中的作用，该突变体延迟了果蝇胚胎外周运动神经的先驱化。在没有运动神经的情况下，成肌细胞正常融合形成多核肌管，肌管与表皮形成正常附着，并产生并维持与野生型模式相当的幼虫肌肉组织。同样，预示成虫肌肉组织的表达twist的成肌细胞在没有运动神经的情况下正常分离，迁移到它们最终的胚胎位置，并继续表达twist直到胚胎发育结束。在没有运动神经的情况下，肌管在正确的发育阶段解偶联形成单个细胞。随后，未受神经支配的肌管发育出幼虫肌肉的成熟电特性和收缩特性，其时间进程与正常受神经支配的肌管无法区分。我们得出结论，神经支配在果蝇胚胎体壁肌肉的模式形成、形态发生、维持或生理发育中不起作用。
