Young A A, Gedulin B, Vine W, Percy A, Rink T J
Amylin Pharmaceuticals Inc., San Diego, California, USA.
Diabetologia. 1995 Jun;38(6):642-8. doi: 10.1007/BF00401833.
Gastric emptying was measured in normal and insulin-treated spontaneously diabetic BB rats using the retention of an acaloric methylcellulose gel containing phenol red delivered by gavage. Dye content in stomachs removed after killing 20 min later was determined spectroscopically, and was compared to that in rats killed immediately after gavage to assess emptying. Diabetic rats had a markedly greater gastric emptying (90.3 +/- 1.7% passed) compared to normal Harlan Sprague Dawley rats (49.1 +/- 4.7% passed; p < 0.001) and non-diabetic BB rats (61.1 +/- 9.2% passed; p < 0.001). The pancreatic beta-cell peptide, amylin, which is deficient in insulin-dependent diabetes mellitus, dose-dependently inhibited gastric emptying in both normal and diabetic rats. The ED50 of the response in normal rats measured by phenol red and novel [3-3H]glucose gavage techniques was approximately 0.4 microgram. This dose was estimated to increase plasma amylin concentration by a mean of approximately 20 pmol/l to concentrations within the range observed in vivo. It is proposed that amylin could participate in the physiological control of nutrient entry into the duodenum and that the accelerated gastric emptying seen in BB rats could be related to their lack of amylin secretion.
采用灌胃给予含酚红的无热量甲基纤维素凝胶并检测其潴留情况，来测定正常和经胰岛素治疗的自发性糖尿病BB大鼠的胃排空。20分钟后处死大鼠，取出胃并通过光谱法测定其中的染料含量，将其与灌胃后立即处死的大鼠的染料含量进行比较，以评估胃排空情况。与正常的哈兰·斯普拉格·道利大鼠（49.1±4.7%排空）和非糖尿病BB大鼠（61.1±9.2%排空；p<0.001）相比，糖尿病大鼠的胃排空明显更快（90.3±1.7%排空）。胰腺β细胞肽胰淀素在胰岛素依赖型糖尿病中缺乏，它在正常和糖尿病大鼠中均呈剂量依赖性地抑制胃排空。通过酚红和新型[3-3H]葡萄糖灌胃技术测定，正常大鼠反应的半数有效剂量（ED50）约为0.4微克。该剂量估计可使血浆胰淀素浓度平均升高约20皮摩尔/升，达到体内观察到的浓度范围。有人提出，胰淀素可能参与营养物质进入十二指肠的生理控制，并且在BB大鼠中观察到的胃排空加速可能与其胰淀素分泌缺乏有关。
