Taga M, Sakakibara H, Saji M, Minaguchi H
Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Japan.
Horm Res. 1995;44 Suppl 2:23-9. doi: 10.1159/000184657.
In order to clarify a regulatory mechanism of epidermal growth factor (EGF) in the decidualization process, EGF gene expression, the change in EGF receptor, and the cell proliferative effect of EGF in the human endometrium/decidua were assessed in both in vivo and in vitro decidualization systems. Northern blot hybridization showed that, although no hybridized band was found in the proliferative and secretory phase endometria, a specific band of 5 kb was detected in decidua of early pregnancy as well as in in vitro medroxyprogesterone acetate-induced decidual cells. In situ hybridization revealed that prepro-EGF mRNA was observed in the stromal cells of decidua. Scatchard plot analysis of 125I-EGF-binding studies for the homogenates from these endometria/decidua revealed that its binding sites increased in accordance with the decidualization process, and its dissociation constant remained unchanged. EGF had a stimulatory action on cell proliferation in the early pregnant decidual cells. These results demonstrate that EGF production, EGF receptor, and decidual cell growth increase in accordance with the process of decidualization, suggesting that EGF may play an important role in the regulation of decidualization in human endometrium through an autocrine/paracrine system.
为阐明表皮生长因子（EGF）在蜕膜化过程中的调控机制，我们在体内和体外蜕膜化系统中评估了人子宫内膜/蜕膜中EGF基因表达、EGF受体的变化以及EGF对细胞增殖的影响。Northern印迹杂交显示，虽然在增殖期和分泌期子宫内膜中未发现杂交带，但在早孕蜕膜以及体外醋酸甲羟孕酮诱导的蜕膜细胞中检测到一条5 kb的特异性条带。原位杂交显示，在蜕膜的基质细胞中观察到前体EGF mRNA。对这些子宫内膜/蜕膜匀浆进行的125I-EGF结合研究的Scatchard图分析表明，其结合位点随着蜕膜化过程而增加，其解离常数保持不变。EGF对早孕蜕膜细胞的细胞增殖有刺激作用。这些结果表明，随着蜕膜化过程，EGF的产生、EGF受体和蜕膜细胞生长增加，提示EGF可能通过自分泌/旁分泌系统在人子宫内膜蜕膜化的调节中发挥重要作用。
