Puche A C, Key B
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
J Comp Neurol. 1995 Jul 10;357(4):513-23. doi: 10.1002/cne.903570403.
Interactions between carbohydrate ligands and their receptors play an important role in cell adhesion and migration in many tissues. Cell-surface carbohydrates that contain terminal galactose have previously been implicated in primary sensory axon growth in the rodent olfactory system. The aim of the present study was to determine whether galectin-1, a galactose-binding receptor, was expressed within the rat primary olfactory pathway. Immunohistochemical and in situ hybridisation analyses revealed expression of galectin-1 by primary sensory olfactory neurons during the major embryonic period of axonogenesis as well as in maturity. In the adult olfactory bulb, galectin-1 was expressed by both second-order projection neurons and interneurons and was selectively localised to the synaptic neuropil layers. Mitral cells, the principal postsynaptic target of primary olfactory axons, began expressing this lectin soon after genesis and maintained high levels into adulthood. The expression of galectin-1 in the primary olfactory pathway and olfactory bulb neuropil suggests a role for this lectin both in the initial formation and in the subsequent maintenance of neuronal connections between the peripheral and the central olfactory neurons as well as between neurons within the bulb.
碳水化合物配体与其受体之间的相互作用在许多组织的细胞黏附和迁移中发挥着重要作用。含有末端半乳糖的细胞表面碳水化合物先前已被证明与啮齿动物嗅觉系统中初级感觉轴突的生长有关。本研究的目的是确定半乳糖凝集素-1（一种半乳糖结合受体）是否在大鼠初级嗅觉通路中表达。免疫组织化学和原位杂交分析显示，在轴突发生的主要胚胎期以及成熟阶段，初级感觉嗅觉神经元均表达半乳糖凝集素-1。在成年嗅球中，二阶投射神经元和中间神经元均表达半乳糖凝集素-1，且选择性地定位于突触神经毡层。初级嗅觉轴突的主要突触后靶标——二尖瓣细胞，在生成后不久就开始表达这种凝集素，并在成年后维持高水平表达。半乳糖凝集素-1在初级嗅觉通路和嗅球神经毡中的表达表明，这种凝集素在周围和中枢嗅觉神经元之间以及嗅球内神经元之间神经元连接的初始形成和后续维持中均发挥作用。
