Mills P C, Ng J C, Seawright A A, Auer D E
Department of Veterinary Pathology, University of Queensland, St. Lucia, Australia.
J Vet Pharmacol Ther. 1995 Jun;18(3):204-9. doi: 10.1111/j.1365-2885.1995.tb00579.x.
Vascular leakage induced by intradermal injection of endotoxin, zymosan-activated plasma (ZAP) and platelet-activating factor (PAF) was measured in nine Thoroughbreds using 125-iodine human serum albumin (125I-HSA) as a marker in the blood. ZAP and PAF produced dose-dependent increases in vascular permeability with the maximum occurring within the first 15 min after injection. The vascular leakage induced by endotoxin was also dose-dependent, but the maximum occurred 2 h after intradermal injection. Intradermal sites previously injected with endotoxin were refractory to a second injection of endotoxin for up to 5 days. However, sites injected with endotoxin and re-injected with either ZAP or PAF remained responsive with increased vascular leakage compared to saline injected control sites re-injected with either ZAP or PAF. Diminished response to endotoxin challenge may contribute to the poor prognosis of endotoxaemia in the horse.
在内皮素、酵母聚糖激活血浆（ZAP）和血小板激活因子（PAF）皮内注射诱导的血管渗漏实验中，以125碘人血清白蛋白（125I-HSA）作为血液中的标记物，对9匹纯种马进行了测量。ZAP和PAF使血管通透性呈剂量依赖性增加，在注射后的前15分钟内达到最大值。内毒素诱导的血管渗漏也呈剂量依赖性，但在皮内注射后2小时达到最大值。先前注射过内毒素的皮内部位在长达5天的时间内对第二次内毒素注射无反应。然而，与注射生理盐水的对照部位再次注射ZAP或PAF相比，注射内毒素后再注射ZAP或PAF的部位血管渗漏增加，仍有反应。对内毒素攻击反应减弱可能导致马内毒素血症预后不良。
