Yukawa N, Takahama K
Department of Legal Medicine, Miyazaki Medical College, Japan.
Nihon Hoigaku Zasshi. 1995 Jun;49(3):222-8.
Using 2 kitchen knives, 2 stab wounds were made into the abdomen of a pig that had been sacrificed under sanctioned processing methods of a meat packing plant. One knife wound penetrated the jejunum and the other, the liver. After leaving the knives at room temperature for a week, the stains on the blades were extracted and subjected to a sandwich enzyme immunoassay to detect the presence of sucrase-isomaltase (sucrase-alpha-dextrinase or SI), a major digestive enzyme of the small intestine. Results of this assay revealed that sufficient SI could be detected only on the knife that had penetrated the jejunum. This animal experiment thus suggests the potential usefulness of an SI assay to identify a weapon that has penetrated the small intestine. Further investigation will be pursued to determine whether this detection method is also valid in humans.
使用两把菜刀，在一家肉类加工厂经批准的加工方法宰杀的猪的腹部制造了两处刺伤。一处刀伤穿透空肠，另一处穿透肝脏。将刀具在室温下放置一周后，提取刀刃上的污渍，并进行夹心酶免疫测定，以检测蔗糖酶异麦芽糖酶（蔗糖酶-α-糊精酶或SI）的存在，蔗糖酶异麦芽糖酶是小肠的一种主要消化酶。该测定结果显示，仅在穿透空肠的刀具上能检测到足够的蔗糖酶异麦芽糖酶。因此，该动物实验表明蔗糖酶异麦芽糖酶测定在识别穿透小肠的凶器方面具有潜在用途。将进一步开展研究以确定这种检测方法在人体中是否也有效。
